hybez ii hybridization system oven  (Advanced Cell Diagnostics Inc)

Journal: STAR Protocols

Article Title: Analysis of target mRNAs in the fixed-frozen human brain using a modified BaseScope-ISH Assay protocol

doi: 10.1016/j.xpro.2022.101896

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Article Snippet: HybEZTM II Hybridization System (from now on referred to as “HybEZ oven”) , ACDBio , 321710 and 321720.

Techniques: Hood, Plasmid Preparation, RNAscope, Software, Staining, Hybridization

Journal: Frontiers in Molecular Biosciences

Article Title: Adamts10 controls transforming growth factor β family signaling that contributes to retinal ganglion cell development

doi: 10.3389/fmolb.2022.989851

Figure Lengend Snippet: Ocular expression of adamts10 mRNA in zebrafish. (A) Expression of adamts10 mRNA (red dots, top row) was observed along the retinal pigment epithelium (dark brown/black structure) and dispersed throughout the retina of embryos at 24, 48, and 72 hpf. In 72 hpf eyes, robust signal (open arrowheads) extending from the inner retina and traversing a gap in the retinal pigmented epithelium (blue arrows, insert) is suggestive of optic nerve expression of adamts10 mRNA. (B) In 2-year-old adult eyes, expression of adamts10 mRNA was found in the outer nuclear layer (ONL), inner nuclear layer (INL), and ganglion cell layer (GCL). Hybridization probe against DapB , a bacterial gene, served as negative control on adjacent sections (bottom rows, A and B ). Scale bar: 50 µm. L, Lens.

Article Snippet: Following 30 min protease plus treatment at 40°C in a hybridization oven (HybEZ II Hybridization System, Advanced Cell Diagnostics), samples were hybridized with probes, subjected to signal amplification steps and then reacted with Fast Red.

Techniques: Expressing, Hybridization, Negative Control